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Simulation consideration Many sequential circuit examples in the book operate at a relatively slow rate, as does the enable pulse of the LED time-multiplexing circuit. This can be done by generating a single-clock enable tick from a counter. An 18-bit counter is used in this circuit:

16.21

(14.8)

The most common naming convention for the node names when working with XML is camel-case. Camel-case is starting the first word with a lowercase character and then the first letter of every word after that is uppercase.

12

Command Getting Started and Tutorials Using Dreamweaver Using ColdFusion Reference Extensions Extending Dreamweaver Extensions API Reference Extensions Creating and Submitting Dreamweaver Support Center Macromedia Online Forums Activation Activate Product Activation Transfer License Activation Online Registration Activation Print Registration Description Opens the overview that also includes various tutorials Opens the Dreamweaver online Help system Opens the online Help for ColdFusion Opens the Reference panel to the selected code, if any Opens the general Extending Dreamweaver online documentation Displays the detailed Dreamweaver API reference Windows N/A F1 Ctrl+F1 Shift+F1 N/A Macintosh N/A F1 Command+F1 Shift+F1 N/A

The PHP responsible for loading the banner ad is now complete. As you can see, the amount of code needed to create this application is fairly small. This basic example can easily be extended to add in categories or even multiple images that transition as the movie sits on a browser.

4-5.1.3 Optical Probe. Optical probes can also be used to measure solid concentration and are generally of two types: light absorption or light scattering. In both cases the amount of light absorbed or scattered is related to the solids concentration. For absorption, Beer s law applies:

Electrophoresis is the movement of charged molecules through a solid-phase medium under the in uence of an electrical potential difference In molecular biology the solid-phase media are usually agarose or polyacrylamide gels Agarose is a naturally occurring heteroglycan, which has been described previously and is used for agarose gel electrophoresis (AGE) (Figure 310) Polyacrylamide is a synthetic polyamide polymer that provides the essential solid-phase medium for polyacrylamide gel electrophoresis (PAGE) (see s 2 and 9) AGE is generally used for the separation of larger polydeoxynucleotides/polynucleotides (>100 bases), whereas PAGE is frequently used for the separation of smaller polydeoxynucleotides/polynucleotides or oligodeoxynucleotides/oligonucleotides (<100 bases) Therefore, for the separation of large nucleic acids, agarose is generally the solid-phase medium of choice (Figure 310) Agarose gels are very simple to make when compared with polyacrylamide gels.

Initially, agarose is mixed with either tris acetic acid/ethylenediaminetetraacetic acid (TAE) or tris boric acid/ethylenediaminetetraacetic acid (TBE) buffer, then the heterogenous mixture is heated to dissolve the agarose, and cooled to gelation in a gel-slab mounted with a plastic comb to create sample wells TAE results in larger agarose pore sizes than TBE Hence TAE gels are generally used to resolve DNA larger than 1000 bases, and TBE gels to resolve smaller DNA fragments However, recovery of DNA is generally poorer from TBE agarose gels, and therefore TAE often ends up being used for the resolution of smaller DNA fragments too In order to run an agarose gel, samples are mixed with a loading buffer, which contains glycerol or Ficoll to increase the density of the sample so that samples sink easily into the wells, plus mobility dyes to aid sample loading and monitoring of the electrophoretic process.

After electrophoresis, DNA may be visualised by staining with ethidium bromide (Figure 311) Ethidium bromide is a base pair intercalator that inserts or intercalates between base pairs (maximum 1 ethidium bromide/65 base pairs) and in so doing acquires a substantial increase in F and hence uorescence intensity at Imax (595 nm) (see 4) Ethidium bromide is a powerful mutagen Hence other staining procedures have been devised These include silver, methylene blue or acridine orange staining Silver staining has a similar sensitivity to ethidium bromide staining, but the former is able to give a permanent record of the gel, unlike the latter Methylene blue staining is 40 times less sensitive than ethidium bromide, but causes less damage to DNA and is non-carcinogenic Acridine orange is useful for differentiating single-stranded (ss) and double-stranded (ds) DNA since dsDNA will uoresce green under UV light whilst ssDNA will uoresce red.

The recovery of DNA samples and fragment from electrophoresis gels is relatively simple There were originally a wide range of methods to recover DNA from agarose gels, but methods have frequently been superseded by the use of the GuNCS miniprep method The approach in this case here is literally to cut out the DNA.